Journal: MethodsX

Article Title: Around the ThermoClock : A precision automated temperature control system for Ex Vivo circadian studies

doi: 10.1016/j.mex.2025.103717

Figure Lengend Snippet: ThermoClock system overview schematics. The microcontroller (Arduino DUE) receives the desired temperature vector, T desired ( t ) , which contains setpoints of each temperature module, T desired , i ( t ) . It also the measured temperature array, T measured ( t ), which contains real-time temperature readings from each module, T measured , i ( t ) . The microcontroller calculates the error array, ( t ) , which is the input to the proportional-integral-derivative (PID) control operating under the same gains for every module. The PID control output PWM signals, u i ( t ) , which are sent to individual bipolar junction transistors (BJTs). BJTs regulate the current output, i i ( t ) , to the heating pads, thereby controlling their temperatures and that of the well plates placed on top. The actual temperature of each module is measured using a resistance temperature detector (RTD), then sent back to the microcontroller for calculation and for logging onto a microSD card.

Article Snippet: Platinum RTD Sensor - PT100 - 3 Wire 1 m long , 5 , Adafruit , 59.75 , .

Techniques: Plasmid Preparation, Control